calu-1(tm1783) x  (BioResource International Inc)

Journal: Biology

Article Title: Calumenin, a Ca 2+ Binding Protein, Is Required for Dauer Formation in Caenorhabditis elegans

doi: 10.3390/biology12030464

Figure Lengend Snippet: Dauer formation assay of the calu-1 ( tm1783 ) mutant. ( a ) Schematic diagram of the dauer formation assay. Four independent assays were performed, each in duplicate or triplicate, using ascr#2 ( b ) or crude dauer pheromone ( c ). Mean ratios with SEM (standard error of mean) are represented in bar graphs. ***, p < 0.001; *, p < 0.05; ns, not significant.

Article Snippet: The following strains were used in this study: Bristol N2 (wild type), calu-1 ( tm1783 ) X, CF1038 daf-16 ( mu86 ) I, CB1370 daf-2 ( e1370 ) III, BC12836 dpy-5 ( e907 ) /dpy-5 ( e907 ), and sIs11268 (rCes M03F4.7:GFP + pCeh361). calu-1 ( tm1783 ) X was obtained from the National BioResource Project, Japan and others were obtained from the Caenorhabditis Genetics Center (CGC).

Techniques: Tube Formation Assay, Mutagenesis

Journal: International Journal of Molecular Sciences

Article Title: Novel Findings of Anti-Filarial Drug Target and Structure-Based Virtual Screening for Drug Discovery

doi: 10.3390/ijms19113579

Figure Lengend Snippet: Enhanced sensitivity of the calu-1 ( tm1783 ) mutant against ( A ) albendazole (ALB) and ( B ) ivermectin (IVM). A survival test was conducted on food-supplemented nematode growth media (NGM) containing various concentrations of drugs. Survival was measured in 24 h intervals for three days after drug treatment (See Method details). Mean survival rate (%) ± SEM ( n number) was shown in figure and table at three days post-exposure of ALB ( A ) and at two days post-exposure of IVM ( B ). Survival rate was normalized by control (untreated). The p values were evaluated by t -test on both untreated vs. treated and wild type vs. mutant. *** p < 0.001.

Article Snippet: The calu-1 ( tm1783 ) X mutant was obtained from the National BioResource Project, Tokyo, Japan.

Techniques: Mutagenesis, Control

Journal: International Journal of Molecular Sciences

Article Title: Novel Findings of Anti-Filarial Drug Target and Structure-Based Virtual Screening for Drug Discovery

doi: 10.3390/ijms19113579

Figure Lengend Snippet: Resistance of calu-1 ( tm1783 ) mutant against ITC. A survival test was conducted in food-supplemented M9 buffer containing a various concentration of the drug. Survival was measured in 24 h intervals for three days after drug treatment (See Method details). The mean survival rate (%) ± SEM ( n number) are shown in the figure and table at three days post-exposure. Survival rate was normalized by the control (untreated). The p values were evaluated by t -test on both untreated vs. treated and wild type vs. mutant. *** p < 0.001.

Article Snippet: The calu-1 ( tm1783 ) X mutant was obtained from the National BioResource Project, Tokyo, Japan.

Techniques: Mutagenesis, Concentration Assay, Control